I fit my Trek Domane 58cm road bike in the back with the seats down and both wheels off the bike. There is still plenty of room in the back for the wheels, a bag, pump and other things you might bring for a ride. The height of the trunk opening under the rear window is a limiting factor and it was tricky getting 42cm bars through when I put the bike in front-first, but now I put it in rear-first and it's much easier.